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c di gmp levels  (ATCC)
99
ATCC c di gmp levels
Enhanced Psl production at RT was mediated by the <t>c-di-GMP–FleQ</t> pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.
C Di Gmp Levels, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human il 2  (Miltenyi Biotec)
94
Miltenyi Biotec recombinant human il 2
Enhanced Psl production at RT was mediated by the <t>c-di-GMP–FleQ</t> pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.
Recombinant Human Il 2, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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humankine recombinant human hgf protein gmp grade  (Proteintech)
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Proteintech humankine recombinant human hgf protein gmp grade
Enhanced Psl production at RT was mediated by the <t>c-di-GMP–FleQ</t> pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.
Humankine Recombinant Human Hgf Protein Gmp Grade, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1084 gmp  (Proteintech)
94
Proteintech 1084 gmp
Enhanced Psl production at RT was mediated by the <t>c-di-GMP–FleQ</t> pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.
1084 Gmp, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3 cd28 macs gmp t cell transact reagent  (Miltenyi Biotec)
94
Miltenyi Biotec cd3 cd28 macs gmp t cell transact reagent
Enhanced Psl production at RT was mediated by the <t>c-di-GMP–FleQ</t> pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.
Cd3 Cd28 Macs Gmp T Cell Transact Reagent, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant cas9  (TaKaRa)
95
TaKaRa recombinant cas9
Enhanced Psl production at RT was mediated by the <t>c-di-GMP–FleQ</t> pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.
Recombinant Cas9, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c di gmp  (InvivoGen)
96
InvivoGen c di gmp
Enhanced Psl production at RT was mediated by the <t>c-di-GMP–FleQ</t> pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.
C Di Gmp, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c di gmp cdg  (InvivoGen)
96
InvivoGen c di gmp cdg
Enhanced Psl production at RT was mediated by the <t>c-di-GMP–FleQ</t> pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.
C Di Gmp Cdg, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tlrl nacdg  (InvivoGen)
96
InvivoGen tlrl nacdg
Enhanced Psl production at RT was mediated by the <t>c-di-GMP–FleQ</t> pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.
Tlrl Nacdg, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdi gmp bis 3 5  (InvivoGen)
96
InvivoGen cdi gmp bis 3 5
Enhanced Psl production at RT was mediated by the <t>c-di-GMP–FleQ</t> pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.
Cdi Gmp Bis 3 5, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Enhanced Psl production at RT was mediated by the c-di-GMP–FleQ pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.

Journal: The Journal of Biological Chemistry

Article Title: Temperature downshifts induce biofilm formation in Pseudomonas aeruginosa through the SiaABCD signal and functional module

doi: 10.1016/j.jbc.2025.111086

Figure Lengend Snippet: Enhanced Psl production at RT was mediated by the c-di-GMP–FleQ pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.

Article Snippet: LC-MS/MS revealed a reduction in c-di-GMP levels in ATCC 27853 Δ siaD cells compared to the WT ( F ).

Techniques: Activity Assay, Mutagenesis

The DGC SiaD is primarily responsible for the elevated c-di-GMP content and enhanced Psl production at RT in ATCC 27853. A , relative maximal biofilm biomass of 28 isogenic mutants relative to the ATCC 27853 WT at RT. Blue , genes identified in RNA-seq analysis. B , relative maximal biofilm formation of ATCC 27853 and its isogenic Δ siaD strain at RT and 37 °C. C , relative maximal biofilm formation of ATCC 27853, isogenic Δ siaD mutant, and Δ siaD mutant complemented with rhamnose-inducible expression of siaD at 48 h at RT. VC, the pJM253 vector control. D – F , relative activity of the chromosomal P psl - lux transcriptional fusion reporter ( D ), Psl level in biofilm matrix ( E ), and intracellular c-di-GMP level ( F ) in ATCC 27853 and its isogenic Δ siaD mutant at RT. Midlog phase static cells harvested at 24 h at RT were collected for the analysis in ( C–F ). Data are represented as mean ± SD. (A–E), n = 3 independent experiments. ( F ), n = 2 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate; DGC, diguanylate cyclase.

Journal: The Journal of Biological Chemistry

Article Title: Temperature downshifts induce biofilm formation in Pseudomonas aeruginosa through the SiaABCD signal and functional module

doi: 10.1016/j.jbc.2025.111086

Figure Lengend Snippet: The DGC SiaD is primarily responsible for the elevated c-di-GMP content and enhanced Psl production at RT in ATCC 27853. A , relative maximal biofilm biomass of 28 isogenic mutants relative to the ATCC 27853 WT at RT. Blue , genes identified in RNA-seq analysis. B , relative maximal biofilm formation of ATCC 27853 and its isogenic Δ siaD strain at RT and 37 °C. C , relative maximal biofilm formation of ATCC 27853, isogenic Δ siaD mutant, and Δ siaD mutant complemented with rhamnose-inducible expression of siaD at 48 h at RT. VC, the pJM253 vector control. D – F , relative activity of the chromosomal P psl - lux transcriptional fusion reporter ( D ), Psl level in biofilm matrix ( E ), and intracellular c-di-GMP level ( F ) in ATCC 27853 and its isogenic Δ siaD mutant at RT. Midlog phase static cells harvested at 24 h at RT were collected for the analysis in ( C–F ). Data are represented as mean ± SD. (A–E), n = 3 independent experiments. ( F ), n = 2 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate; DGC, diguanylate cyclase.

Article Snippet: LC-MS/MS revealed a reduction in c-di-GMP levels in ATCC 27853 Δ siaD cells compared to the WT ( F ).

Techniques: RNA Sequencing, Mutagenesis, Expressing, Plasmid Preparation, Control, Activity Assay

A model to illustrate the activation of SiaABCD and its mediated biofilm enhancement in ATCC 27853 in response to temperature downshifts. Temperature downshift from 37 °C to RT causes membrane perturbations in ATCC 27853, which induce SiaA phosphatase activity to promote the formation of SiaC–SiaD complex and DGC activity of SiaD. The activated Sia system regulates psl production via c-di-GMP–FleQ–Psl pathway. Increased Psl production enhanced biofilm formation of ATCC 27853 at RT through enhancing both surface attachment and biofilm matrix development. c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate; DGC, diguanylate cyclase.

Journal: The Journal of Biological Chemistry

Article Title: Temperature downshifts induce biofilm formation in Pseudomonas aeruginosa through the SiaABCD signal and functional module

doi: 10.1016/j.jbc.2025.111086

Figure Lengend Snippet: A model to illustrate the activation of SiaABCD and its mediated biofilm enhancement in ATCC 27853 in response to temperature downshifts. Temperature downshift from 37 °C to RT causes membrane perturbations in ATCC 27853, which induce SiaA phosphatase activity to promote the formation of SiaC–SiaD complex and DGC activity of SiaD. The activated Sia system regulates psl production via c-di-GMP–FleQ–Psl pathway. Increased Psl production enhanced biofilm formation of ATCC 27853 at RT through enhancing both surface attachment and biofilm matrix development. c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate; DGC, diguanylate cyclase.

Article Snippet: LC-MS/MS revealed a reduction in c-di-GMP levels in ATCC 27853 Δ siaD cells compared to the WT ( F ).

Techniques: Activation Assay, Membrane, Activity Assay